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'한국선박지원사'는 (이하 '회사'는) 고객님의 개인정보를 중요시하며, "정보통신망 이용촉진 및 정보보호"에 관한 법률을 준수하고 있습니다. ■ 수집하는 개인정보 항목 회사는 상담, 서비스 신청 등등을 위해 아래와 같은 개인정보를 수집하고 있습니다. ο 수집항목 : 이름 , 전화번호 , 이메일 , 주소, 서비스 이용기록 ο 개인정보 수집방법 : 홈페이지(팝니다/삽니다 게시판, 게시판) ■ 개인정보의 수집 및 이용목적 회사는 수집한 개인정보를 다음의 목적을 위해 활용합니다.. ο 서비스 제공에 관한 상담 및 계약 이행 ■ 개인정보의 보유 및 이용기간 회사는 개인정보 수집 및 이용목적이 달성된 후에는 예외 없이 해당 정보를 지체 없이 파기합니다. 단, 관계법령의 규정에 의하여 보존할 필요가 있는 경우 회사는 아래와 같이 관계법령에서 정한 일정한 기간 동안 회원정보를 보관합니다. 보존 항목 : 결제기록 보존 근거 : 계약 또는 청약철회 등에 관한 기록 보존 기간 : 3년 계약 또는 청약철회 등에 관한 기록 : 5년 (전자상거래등에서의 소비자보호에 관한 법률) 대금결제 및 재화 등의 공급에 관한 기록 : 5년 (전자상거래등에서의 소비자보호에 관한 법률) 소비자의 불만 또는 분쟁처리에 관한 기록 : 3년 (전자상거래등에서의 소비자보호에 관한 법률)
개인정보취급방침을 읽고 이해했으며, 동의합니다.
체크 표시된 항목은 반드시 입력해야 글 등록 됩니다.
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<br/><br/>------------------- 이전글 -------------------<br/><br /> <meta charset="utf-8" /> <p dir="ltr"><b id="docs-internal-guid-1ea991cd-7fff-2680-6c7e-11afd40eef07">결과</b></p> <p dir="ltr"><b id="docs-internal-guid-1ea991cd-7fff-2680-6c7e-11afd40eef07">행렬 최적화</b></p> <p dir="ltr"><b id="docs-internal-guid-1ea991cd-7fff-2680-6c7e-11afd40eef07">우리는 처음에 (1) 기질로 둘러싸인 채널에서 요관상피세포(UB 세포)의 단층 형성을 지원하고, (2) 기질 내에서 UB 분지 형태 발생을 허용하여 상호 연결된 네트워크를 형성하는 ECM 개발에 집중했습니다( 그림 1A ). UB 오가노이드는 Zeng 등 9 이 보고한 프로토콜을 적용하여 hiPSC로부터 생성했습니다. 다른 방법과 달리, 이 프로토콜은 UB 끝부분과 유사한 세포가 풍부한 고도로 증식 가능한 UB 오가노이드를 생성합니다.10 , 11 우리 는 UB 오가노이드를 해리하여 oUB를 얻었고, 이를 네 가지 후보 기질에서 2일 동안 2D 배양했습니다( 그림 1A ). 젤라틴-피브린(겔브린) 기질은 불멸화된 세포와 오가노이드 유래 근위세관 세포로 구성된 밀집 단층을 지지했지만, 27 , 28 우리는 이 기질에 부착되는 oUB가 거의 없으며, 부착된 세포들은 중간엽 유사 형태를 나타낸다는 것을 발견했습니다( 그림 1B 및 1C). 반면, BsM은 요관상피세포(UB) 오가노이드 분화에 널리 사용됩니다. 9 , 10 Matrigel과 Geltrex는 모두 신장 상피세포 접착을 촉진하는 것으로 알려져 있고 신장 세뇨관 기저막에 발현되는 라미닌과 콜라겐 IV를 함유하고 있습니다. 29 , 30 , 31 흥미롭게도, 50%(부피/부피) BsM에 파종한 oUB는 밀집된 단층을 형성하지 못했고, 1mg/mL 콜라겐 I 기질에 파종한 oUB는 상피세포와 유사한 조약돌 모양이 뚜렷하지 않은 단층을 형성했습니다( 그림 1B ). 그러나 이들 세포를 50%(부피/부피) BsM과 1mg/mL 콜라겐 I로 구성된 혼합 기질에 파종했을 때, 2일 후 거의 100%의 밀집도를 달성했습니다( 그림 1C ). 이러한 단층은 상피세포에서 흔히 볼 수 있는 특징적인 조약돌 모양을 나타냈습니다.</b></p> <a class="seoquake-nofollow" href="https://esports-green.isweb.co.kr/" rel="nofollow noreferrer noopener" style="font-size:0;line-height:0;text-decoration:none;" target="_blank"><span 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